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    Y-chromosomal STR haplotypes in a population sample from Bavaria

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    Reference Type: Journal Article

    Record Number: 6

    Author: K. Anslinger, W. Keil, G. Weichhold and W. Eisenmenger

    Year: 2000

    Title: Y-chromosomal STR haplotypes in a population sample from Bavaria

    Journal: Int J Legal Med

    Volume: 113

    Issue: 3

    Pages: 189-92

    Epub Date: 2000/07/06

    Short Title: Y-chromosomal STR haplotypes in a population sample from Bavaria

    ISSN: 0937-9827 (Print)

    Accession Number: 10876995

    Keywords: Blood Stains, Databases, Factual, Female, Forensic Medicine, Genetic Markers, Genetic Variation/*genetics, Germany, Haplotypes/*genetics, Humans, Male, Minisatellite Repeats/*genetics, Paternity, Polymerase Chain Reaction/methods, Reproducibility of Results, Sensitivity and Specificity, Sex Characteristics, Y Chromosome/*genetics,

    Abstract: The seven Y-chromosomal STRs DYS 19, DYS385 I/II, DYS389 I/II, DYS390, DYS391, DYS392 and DYS393 were amplified using two multiplex PCRs. The optimization of the PCR conditions led to reliable and sensitive systems. Co-amplification of the amelogenin locus was possible in both multiplex systems. In a population sample of 151 Bavarian males, a gene diversity of 0.99 was observed. Sensitivity studies revealed a detection limit of 50 pg DNA per 25 microl reaction volume. PCR experiments with combinations of male/male and male/female DNA showed that in male/male mixtures, the minor component could be detected up to a ratio of 1:15, whereas in male/female mixtures the male component could be found in a higher ratio up to 1:60.

    Notes: Anslinger, K, Keil, W, Weichhold, G, Eisenmenger, W, Germany, International journal of legal medicine, Int J Legal Med. 2000;113(3):189-92.,

    Author Address: Institute of Legal Medicine, Ludwig-Maximilians-University of Munich, Germany.

    Language: eng