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    A forensic method for the simultaneous analysis of biallelic markers identifying Y chromosome haplogroups inferred as having originated in Asia and the Japanese archipelago


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    Reference Type: Journal Article

    Record Number: 7479

    Author: N. Mizuno, T. Kitayama, K. Fujii, H. Nakahara, K. Yoshida, K. Sekiguchi, N. Yonezawa, M. Nakano and K. Kasai

    Year: 2010

    Title: A forensic method for the simultaneous analysis of biallelic markers identifying Y chromosome haplogroups inferred as having originated in Asia and the Japanese archipelago

    Journal: Forensic Sci Int Genet

    Volume: 4

    Issue: 2

    Pages: 73-9

    Epub Date: 2010/02/05

    Date: Feb

    Short Title: A forensic method for the simultaneous analysis of biallelic markers identifying Y chromosome haplogroups inferred as having originated in Asia and the Japanese archipelago

    ISSN: 1878-0326 (Electronic)

    DOI: S1872-4973(09)00091-X [pii], 10.1016/j.fsigen.2009.06.001,

    Accession Number: 20129464

    Keywords: Asian Continental Ancestry Group/genetics, Base Sequence, Chromosomes, Human, Y/*genetics, DNA/blood/genetics, DNA Mutational Analysis, DNA Primers, Female, Forensic Medicine/methods, Genetic Markers, Geography, Humans, Japan, Male, Microsatellite Repeats/genetics, Mouth Mucosa, Mutation, Polymerase Chain Reaction/methods, Polymorphism, Genetic,

    Abstract: Information regarding the ancestral and geographical origins of biological evidence samples may be useful for crime investigators as they narrow down the possible donors of the sample. A method for simultaneous analysis of seven biallelic markers (M130, M131, M57, M125, M175, M122 and M134) was developed for forensic application. M57, M125 and M131 are included to identify haplogroups inferred as having originated in the Japanese archipelago. Our method employs allele-specific PCR and fragment analysis using fluorescently labeled primers and capillary electrophoresis. This method can be used to assign a haplogroup from both of degraded male DNA samples and DNA samples containing a mixture of female and male DNA by designing PCR primers that generate small amplicons and are highly specific for targets on the Y chromosome. A total of 1346 samples from Japanese males collected from the four major islands and Okinawa island were classified into seven Y binary haplogroups i.e., C-M130, C-M131, D-M57, D-M125, O-M175, O-M122 and O-M134, and a "no-mutation detected" group and their frequencies were 0.0617, 0.0565, 0.1441, 0.182, 0.3418, 0.11, 0.0847 and 0.0193, respectively. Samples of "no-mutation detected" were further analyzed by direct sequencing for identification of the major haplogroup to which they belong. Along with the haplogroup data, we report haplotype data for the 16 Y-STR markers included in the AmpFlSTR Yfiler PCR amplification kit (Applied Biosystems). These data will be useful in the prediction of haplogroups based on Y-STR haplotypes.

    Notes: Mizuno, Natsuko, Kitayama, Tetsushi, Fujii, Koji, Nakahara, Hiroaki, Yoshida, Kanako, Sekiguchi, Kazumasa, Yonezawa, Naoto, Nakano, Minoru, Kasai, Kentaro, Netherlands, Forensic science international. Genetics, Forensic Sci Int Genet. 2010 Feb;4(2):73-9. Epub 2009 Jul 3.,

    Author Address: National Research Institute of Police Science, 6-3-1 Kashiwanoha, Kashiwa, 277-0882, Japan. mizuno@nrips.go.jp

    Language: eng